| plasmid ID |
|
|
|
| pET-32a (+) |
|
T7 promoter, pBR322 ori, Ampr
|
Novagen |
| pETDuet-1 |
|
double T7 promoters, pBR322 ori, Ampr
|
Novagen |
| pRSFDuet-1 |
|
double T7 promoters, RSF1030 ori, Kanr
|
Novagen |
| pCDFDuet-1 |
|
double T7 promoters, CloDF13 ori, Strepr
|
Novagen |
| pGH1 |
pETDuet-trGtF3′H-trCPR |
pETDuet-1 carrying trGtF3′H and trCPR |
(15) |
| pGH2 |
pETDuet-trGtF3′H-CPR |
pETDuet-1 carrying
trGtF3′H and CPR |
(15) |
| pGH3 |
pETDuet-trLbF3′H-trCPR |
pETDuet-1 carrying trLbF3′H and trCPR |
this study |
| pGH4 |
pETDuet-trLbF3′H-CPR |
pETDuet-1 carrying trLbF3′H and CPR |
this
study |
| pGH5 |
pETDuet-trCnF3′H-trCPR |
pETDuet-1 carrying trCnF3′H and trCPR |
this study |
| pGH6 |
pETDuet-trCnF3′H-CPR |
pETDuet-1 carrying trCnF3′H and CPR |
this
study |
| pGH7 |
pETDuet-trCaF3′H-trCPR |
pETDuet-1 carrying trCaF3′H and trCPR |
this study |
| pGH8 |
pETDuet-trCaF3′H-CPR |
pETDuet-1 carrying trCaF3′H and CPR |
this
study |
| pGH9 |
pETDuet-trThF3′H-trCPR |
pETDuet-1 carrying trThF3′H and trCPR |
this study |
| pGH10 |
pETDuet-trThF3′H-CPR |
pETDuet-1 carrying trThF3′H and CPR |
this
study |
| pGH11 |
pETDuet-trNtF3′H-trCPR |
pETDuet-1 carrying trNtF3′H and trCPR |
this study |
| pGH12 |
pETDuet-trNtF3′H-CPR |
pETDuet-1 carrying trNtF3′H and CPR |
this
study |
| pGH13 |
pETDuet-trSlF3′H-trCPR |
pETDuet-1 carrying trSlF3′H and trCPR |
this study |
| pGH14 |
pETDuet-trSlF3′H-CPR |
pETDuet-1 carrying trSlF3′H and CPR |
this
study |
| pGH15 |
pETDuet-trBfF3′H-trCPR |
pETDuet-1 carrying trBfF3′H and trCPR |
this study |
| pGH16 |
pETDuet-trBfF3′H-CPR |
pETDuet-1 carrying trBfF3′H and CPR |
this
study |
| pGH17 |
pETDuet-trCmF3′H-trCPR |
pETDuet-1 carrying trCmF3′H and trCPR |
this study |
| pGH18 |
pETDuet-trCmF3′H-CPR |
pETDuet-1 carrying trCmF3′H and CPR |
this
study |
| pGH19 |
pET32a-MpOMT |
pET32a carrying MpOMT |
(15) |
| pGH20 |
pET32a-MpOMTS142V
|
pET32a carrying MpOMTS142V
|
this study |
| pGH21 |
pETDuet-trThF3′H-CPR-MpOMTS142V
|
pETDuet-1 carrying trThF3′H, CPR
and MpOMTS142V
|
this study |
| pGH22 |
pRSFDuet-metA-CysE |
pRSFDuet-1
carrying metA and CysE |
this study |
| pGH23 |
pRSFDuet-metA-CysE-ydaO |
pRSFDuet-1
carrying metA, CysE and ydaO |
this study |
| pGH24 |
pRSFDuet-metA-CysE-metK |
pRSFDuet-1
carrying metA, CysE and metK |
this study |
| pGH25 |
pRSFDuet-metA-CysE-ydaO-metK |
pRSFDuet-1 carrying metA, CysE, ydaO and metK |
this
study |
| pGH26 |
pRSFDuet-metK |
pRSFDuet-1 carrying metK |
this study |
| pGH27 |
pETDuet-trThF3′H-CPR-MpOMTS142V-metK |
pETDuet-1 carrying trThF3′H,
CPR, MpOMTS142V and metK |
this study |
| pGH28 |
pRSFDuet-PhCHS |
pRSFDuet-1 carrying PhCHS |
this study |
| pGH29 |
pRSFDuet-FjTAL-PhCHS |
pRSFDuet-1
carrying FjTAL and PhCHS |
this study |
| pGH30 |
pCDFDuet-Pc4CL-MsCHI |
pCDFDuet-1
carrying Pc4CL and MsCHI |
this study |
| pGH31 |
pETDuet-trThF3′H-CPR-MpOMT |
pETDuet-1 carrying trThF3′H, CPR, MpOMT |
this study |
| Strain ID |
|
|
|
|
E. coli BL21(DE3) |
|
|
|
| HE00 |
BL21(DE3) carrying pGH28 and pGH30 |
|
this study |
| HE01 |
BL21(DE3) carrying pGH29 and pGH30 |
|
this study |
| HE02 |
BL21(DE3)
carrying pGH29, pGH30 and pGH31 |
|
this
study |
| HE03 |
BL21(DE3) carrying
pGH21, pGH29 and pGH30 |
|
this study |
| HE04 |
BL21(DE3) carrying pGH27, pGH29
and pGH30 |
|
this study |
