Figure 2.

PEG-lipid characteristics affect LNP structure. (A) SAXS analysis of 5 LNPs with different PEG-lipids composition. The red and blue dashed lines indicate the H_II and L_α signal positions as assigned in Figure 1A. Primary and secondary H_II diffraction peaks at q ∼ 0.11 and ∼ 0.18 Å^–1, respectively, potentially associated with the crystalline phase, are highlighted with orange dashed lines. (B) Cryo-EM images of LNP formulations investigated in panel A. The cryo-EM images highlight the compartmentalization of the ASO-lipid in the H_II (red scale), crystalline H_II (yellow box), L_α (blue scale), and disordered phases (violet circle). ASO-lipid compartmentalization in the H_II phases of some LNPs is highlighted in the zoom-in window.