Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 16;11:1208279. doi: 10.3389/fcell.2023.1208279

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Petrelli, Oztürk, Pind, Ayele, Fainsod and Hicks.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Gsc:Cyp26A1-eGFP is expressed in embryoid bodies induced with activin A and fibroblast growth factor (FGF). (A) Embryoid body (EB) formation was used to generate definitive endoderm germ cells in vitro. Activin A and FGF-2 were used to induce Gsc gene expression, including Cyp26A1-eGFP expression from the targeted Gsc allele. Results clearly demonstrate Gsc:Cyp26A1-eGFP is inducible under these conditions by eGFP expression in Activin A treated cells (middle panels), but not in untreated or EBs generated from wild-type ES cells (left and right panels, respectively). ES cell clones used to generate EBs are identified (C2, H4, G12). (B) Sox17 immunocytochemistry was performed on embryoid bodies to confirm definitive endoderm cell induction (Sox 17). Nuclear staining (DAPI) and overlay (MERGE) are shown. Scale bars: 200 um (A); 40 um (B).