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Purification of yeast iPPase. (A) SDS-PAGE after affinity purification via Ni-NTA. (B) Size exclusion chromatogram purification of pooled elutions from after affinity purification. (C) SDS-PAGE after size exclusion chromatography. (D) RNA preparation using in vitro transcription demonstrates that the laboratory-purified iPPase is active. TF (time final) lanes show a similar amount of RNA being produced with laboratory-purified and the commercially purchased (stock) iPPase.
