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Components and final concentrations of the in vitro transcription reaction.
| Component | Final concentration |
|---|---|
| 5X TRAB | 1 × |
| DTT | 10 mmol/L |
| NTPS | 3 mmol/L |
| GMP | 5 mmol/L |
| iPPase | 0.29 μmol/L (purchased)/0.18 μmol/L (lab) |
| T7 polymerase* | 0.18 μmol/L |
| Ribolock | 0.5% (v/v) |
| Plasmid DNA | 18 ng/μL |
| MilliQ | Up to desired volume |
T7 polymerase produced in-house.
