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. 2023 Jun 16;11:1218099. doi: 10.3389/fbioe.2023.1218099

FIGURE 3.

FIGURE 3

Controlled expression of the catP using a lactose inducible orthogonal TcdR system. The C. acetobutylicum hosts used were either the WT strain (A) or 824BO1 (B), the latter of which carried an integrated copy of tcdR at the pyrE locus under the control of the BgaR::P bgaL lactose inducible promoter system. Strain 824BO1 was transformed with derivatives of the reporter plasmid pMTL82254 (Heap et al., 2009) in which the catP gene was placed either under the control of the P tcdB or P fdx promoter. The WT was transformed with an equivalent plasmid but in which catP was under the control of bgaR::PbgaL. Cells were grown in 2YTG media and induced with lactose after 4 h (downward facing arrow) at concentrations of 0, 1.0, 1.0 and 10 mM. Also included was a C. acetobutylicum negative control carrying the reporter vector pMTL82254 alone with no promoter (None). Experiments were performed in triplicate.