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. 2023 Jun 16;11:1218099. doi: 10.3389/fbioe.2023.1218099

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Copyright © 2023 Omorotionmwan, Wang, Baker, Gizynski, Yoo, Akaluka, Zhang and Minton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acetone and isopropanol producing C. acetobutylicum strain; bearing bgaR-P _bgaL::tcdR (lactose inducible tcdR) at pyrE locus, ctfA/B and adc (encoding CoA transferase and acetoacetate decarboxylase) at purD locus and sadh (encoding a secondary alcohol, dehydrogenase, sADH) at pheA locus. The position of the four loci on the 4.0 Gb C. acetobutylicum chromosome is shown. The bgaR gene encodes the BgaR response regulator which, following binding of lactose brings about transcription of the tcdR gene. The TcdR sigma factor produced promoters binding of RNA polymerase (RNP) to the P_tcdB promoter upstream of sadh at the pheA locus and ctfA/B:adc at the purD locus resulting in production of isopropanol and acetone, respectively.