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. 2023 Jun 28;12(1):2230669. doi: 10.1080/2162402X.2023.2230669

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 6. — Altered murine haematopoiesis during tumor development.

Notes: (a) Pappenheim staining of bone marrow cells in normal, tumor-free and tumor-bearingMsh2^{loxP/loxPVillCre} mice. From each slide, 200 cells were counted and differentiated according to a pre-designed differentiation scheme into the following cell types: myeloblasts, neutrophil pro-/myelocytes, metamyelocytes/neutrophil, eosinophilic pro-/myelocytes, metamyelocytes/eosinophils, lymphocytes, plasma cells, monoblasts, and monocytes. Upper part: representative images of each treatment. Magnification x1000; lower part: Quantification was done blinded by an experienced researcher. n = 7 mice/group. Mean + SD, * p<0.05; ** p<0.01; **** p<0.0001; One-way ANOVA (Sidak’s multiple comparisons test). (b, c) Bone marrow differentiation using multi-color spectral flow cytometry. (b) Diagram of hematopoiesis based on the markers used in this study. (c) A 20-marker panel was applied as described in material and methods. (c) Quantitative analysis of lin⁻c-Kit⁺ precursors according to the diagram shown in Figure 6b. Results show data from 100,000 cells/sample. Mean + SD, each symbol represents one case; § p < 0.05; §§ p < 0.01; §§§§ p < 0.0001; One-way ANOVA (Sidak’s multiple comparisons test).