Table 2.

Summary of synaptic protein quantification measured in postsynaptic densities by Western Blot^a

Protein	Age (mo)	Shank3WT NonTg [mean (SD); n]	Shank3WT 3xTg-AD [mean (SD); n]	Shank3Δex4-9 NonTg [mean (SD); n]	Shank3Δex4-9 3xTg-AD [mean (SD); n]	Kruskal–Wallis test (χ2)	Multivariate analyses
Shank3a	6	14.8 (6.4); 8	22.0 (14.6); 8	7.0 (3.1); 8	7.8 (2.2); 8	0.0032**	Lower in Shank3Δex4-9, p < 0.0001 Lower in older mice, p < 0.05
	12	19.7 (5.3); 8	16.7 (9.7); 7	7.7 (2.5); 8	6.2 (2.0); 8	0.0004***
	18	11.3 (5.8); 9	13.5 (1.7); 8	4.3 (1.7); 7	5.2 (1.9); 8	0.0003***
Shank3c, Shank3d	6	8.6 (2.2); 8	12.5 (7.3); 8	8.6 (2.9); 8	9.6 (3.6); 8	0.4127	Lower in older mice, p < 0.05
	12	10.3 (3.0); 8	10.6 (5.6); 7	8.5 (3.1); 8	8.5 (2.8); 8	0.5367
	18	7.5 (2.2); 9	8.5 (1.3); 8	6.4 (2.3); 7	6.6 (2.3); 8	0.4326
Shank3e	6	5.1 (1.3); 8	7.1 (5.0); 8	5.7 (2.6); 8	5.7 (2.4); 8	0.8728	Lower in older mice, p < 0.01
	12	6.5 (1.8); 8	6.4 (3.0); 7	5.5 (1.6); 8	5.3 (2.1); 8	0.5635
	18	4.3 (1.0); 9	4.7 (0.6); 8	3.5 (1.3); 7	3.9 (1.3); 8	0.2001
Shank1	6	3.6 (3.5); 8	4.8 (5.2); 8	4.2 (3.4); 7	2.7 (2.7); 8	0.8122	Lower in older mice, p < 0.05
	12	4.8 (6.6); 8	2.2 (2.9); 7	4.0 (3.6); 8	2.0 (1.7); 8	0.5598
	18	2.0 (1.9); 9	1.6 (1.6); 8	1.2 (1.5); 7	1.9 (2.3); 8	0.8505
Actin	6	2.3 (1.1); 8	2.7 (1.3); 8	2.1 (0.7); 8	2.0 (0.6); 8	0.6974	NS
	12	2.2 (0.5); 8	2.4 (0.6); 7	2.3 (0.7); 8	2.2 (0.7); 8	0.8678
	18	2.5 (1.0); 9	2.6 (0.9); 8	2.6 (1.1); 7	2.8 (1.1); 8	0.9553
Cortactin	6	2.8 (0.8); 8	3.6 (1.2); 8	2.9 (0.6); 8	3.1 (0.9); 8	0.5418	NS
	12	2.8 (0.7); 8	3.1 (0.6); 7	2.8 (0.5); 8	2.9 (0.5); 8	0.8895
	18	2.8 (0.7); 9	3.0 (0.4); 8	2.4 (1.0); 7	3.0 (1.0); 8	0.3829
Drebrin	6	1.5 (0.5); 8	2.1 (1.1); 8	1.7 (0.8); 8	1.7 (0.9); 8	0.6974	NS
	12	1.4 (0.5); 8	1.5 (0.7); 7	1.8 (1.1); 8	1.6 (0.6); 8	0.9219
	18	1.6 (0.6); 9	1.4 (0.4); 8	1.3 (0.4); 7	1.6 (0.6); 8	0.3297
GAP-43	6	4.5 (1.8); 8	6.0 (3.3); 8	5.2 (2.4); 8	5.1 (2.0); 8	0.8795	NS
	12	5.0 (3.0); 8	4.4 (2.7); 7	5.6 (2.6); 8	4.8 (2.6); 8	0.7553
	18	3.8 (1.7); 9	3.9 (1.9); 8	3.8 (1.2); 7	3.9 (2.0); 8	0.9956
Homer 1	6	3.3 (0.7); 8	4.6 (0.8); 7	3.2 (1.1); 8	4.0 (0.8); 8	0.0200*	Higher in 3xTg-AD, p < 0.01
	12	4.4 (1.2); 8	4.6 (1.7); 7	4.4 (1.3); 8	4.2 (1.9); 8	0.8028
	18	3.6 (0.6); 9	3.8 (1.1); 8	2.8 (0.6); 7	3.1 (0.7); 8	0.0872
Septin 3	6	4.8 (1.5); 8	6.5 (3.2); 8	5.1 (1.4); 8	5.4 (1.7); 8	0.6895	NS
	12	6.0 (1.7); 8	6.1 (2.1); 7	5.7 (1.7); 8	5.8 (1.8); 8	0.9779
	18	5.4 (1.4); 9	5.7 (1.2); 8	5.2 (1.5); 7	5.3 (1.5); 8	0.8709
SNAP25	6	3.7 (1.6); 8	5.0 (3.8); 8	3.8 (1.6); 8	3.4 (1.2); 8	0.7041	NS
	12	3.9 (1.5); 8	4.2 (1.6); 7	3.8 (1.5); 8	3.5 (1.2); 8	0.8749
	18	3.7 (1.2); 9	4.0 (1.5); 8	3.7 (1.7); 7	3.6 (1.7); 8	0.9131
Spectrin	6	2.4 (1.2); 8	3.0 (2.4); 8	2.5 (1.1); 8	2.3 (0.9); 8	0.9538	Lower in older mice, p < 0.05
	12	1.8 (0.6); 8	1.5 (0.5); 7	1.8 (0.6); 8	1.9 (0.8); 8	0.6661
	18	2.1 (0.6); 9	2.1 (0.6); 8	1.9 (0.7); 7	2.1 (0.8); 8	0.8514
Synaptophysin	6	8.3 (5.2); 8	14.6 (10.0); 8	7.7 (5.7); 8	12.2 (9.3); 8	0.3200	Higher in 3xTg-AD, p < 0.05
	12	6.3 (3.2); 8	12.1 (11.6); 7	8.5 (3.6); 8	7.8 (4.1); 7	0.7291
	18	11.7 (5.9); 9	12.9 (3.7); 8	9.3 (5.6); 7	14.0 (10.9); 8	0.7402
PSD95	6	10.8 (2.3); 8	10.0 (3.8); 8	10.2 (2.4); 8	10.5 (2.7); 8	—	—
	12	10.3 (2.2); 8	10.7 (2.0); 7	11.2 (2.4); 8	11.4 (1.9); 8	—
	18	10.5 (1.3); 9	10.5 (1.7); 8	10.8 (1.7); 7	11.2 (1.7); 8	—

^aSynaptic protein levels from the PSD fraction of parietotemporal cortex of Shank3^Δex4-9-3xTg-AD mice were measured by WB. Data were normalized with PSD95 and are mean ± SD followed by the sample size of each group. GAP-43, Growth-associated protein 43; SNAP-25, synaptosomal-associated protein 25 kDa; PSD-95, PSD protein 95.

Statistical analysis: nonparametric Kruskal–Wallis test,

*p < 0.01,

**p < 0.001,

***p < 0.0001; multivariate analysis (for age, Shank3^Δex4-9, and 3xTg-AD genotypes) is also shown.