Table 3.

Quantification of soluble and insoluble amyloid-β in parietotemporal cortex and hippocampus of 3xTg-AD mice^a

	Cortex [mean (SEM)]	3xTg-AD	Shank3Δex4-9-3xTg-AD	p
Soluble fraction (fg/μg of total protein)	Males
	Amyloid-β42	3.91 (0.83)	2.65 (0.19)	0.1893
	Amyloid-β40	1.79 (0.33)	2.24 (0.46)	0.4384
	Ratio amyloid-β42/40	2.48 (0.48)	1.46 (0.34)	0.1417
	Females
	Amyloid-β42	12.96 (0.93)	16.19 (1.18)	0.0494*
	Amyloid-β40	4.45 (0.88)	4.82 (0.61)	0.7469
	Ratio amyloid-β42/40	3.29 (0.80)	3.57 (0.51)	0.7776
Insoluble fraction (fg/mg of total tissue)	Males
	Amyloid-β42	30.83 (2.08)	28.59 (0.62)	0.3341
	Amyloid-β40	72.79 (15.37)	71.52 (11.93)	0.9497
	Ratio amyloid-β42/40	0.41 (0.11)	0.54 (0.13)	0.4958
	Females
	Amyloid-β42	43.22 (2.93)	45.17 (3.75)	0.6884
	Amyloid-β40	98.15 (20.96)	128.69 (31.94)	0.4418
	Ratio amyloid-β42/40	0.61 (0.10)	0.52 (0.07)	0.4862

	Hippocampus [mean (SEM)]	3xTg-AD	Shank3Δex4-9-3xTg-AD	p
Soluble fraction (fg/μg of total protein)	Males
	Amyloid-β42	12.31 (1.32)	11.04 (0.67)	0.4114
	Amyloid-β40	34.06 (8.39)	39.66 (6.21)	0.6036
	Ratio amyloid-β42/40	0.48 (0.11)	0.33 (0.09)	0.4848
	Females
	Amyloid-β42	23.95 (1.81)	32.46 (1.68)	0.0119*
	Amyloid-β40	40.87 (8.92)	47.37 (9.63)	0.6431
	Ratio amyloid-β42/40	0.71 (0.14)	0.72 (0.09)	0.9480
Insoluble fraction (fg/mg of total tissue)	Males
	Amyloid-β42	76.32 (19.53)	66.63 (15.94)	0.7088
	Amyloid-β40	240.52 (74.33)	156.34 (64.97)	0.2343
	Ratio amyloid-β42/40	0.25 (0.04)	0.53 (0.12)	0.0630
	Females
	Amyloid-β42	123.39 (22.87)	89.29 (12.07)	0.2907
	Amyloid-β40	325.29 (57.75)	195.39 (35.28)	0.1318
	Ratio amyloid-β42/40	0.37 (0.02)	0.46 (0.03)	0.0433*

^an = 4-8 per group. Measurements of Aβ₄₀ and Aβ₄₂ were performed by ELISA. Data are mean ± SEM.

Statistical analysis: compared with 3xTg-AD mice without Shank3 deletion within each corresponding tissue, fraction, and sex groups, unpaired t test, two-tailed,

*p < 0.05.