Table 2.

PCR reaction conditions to amplify partial cytb, nad5, β-tub1, β-tub2, and β-tub3 genes of Echinococcus granulosus.

Steps	Cycles	cytb	Nad5	Tub1	Tub2	Tub3
Initial denaturation	1	7 min at 95°C
Denaturation	35	15 s at 95°C	15 s at 95°C	30 s at 95°C	30 s at 95°C	30 s at 95°C
Annealing		40 s at 55°C	30 s at 54°C	30 s at 60°C	30 s at 63°C	30 s at 63°C
Extension step		90 s at 72°C	60 s at 72°C	60 s at 72°C	60 s at 72°C	60 s at 72°C
Final extension	1	7 min at 72°C