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[Preprint]. 2023 Jun 4:2023.06.02.542661. [Version 1] doi: 10.1101/2023.06.02.542661

Figure 4. Treatment with long acting GDF15 influences the response to the anorectic actions of acute GDF15 treatment in mice.

Figure 4

A: Schema of the experimental paradigm. Single housed, adult male and female C57Bl/6J mice were injected with 0.01mg/kg of Fc-GDF15–15 fusion protein (Fc_GDF15) or vehicle control (PBS). Food intake was measured overnight (from 5pm to 9am) before (black bar) and after treatment (red bar) with standard, short acting human recombinant GDF-15 (hrGDF15 0.1mg/kg). Body weight was measured at the same timepoints. B: Food intake recorded overnight (5pm-9am) the day before (black dots) and after an acute bolus of hrGDF15 (red dots) in mice with and without pre-treatment with Fc_GDF15. C: Body weight at 9am the day before (black dots) and 9 am the day after (red dots) an acute bolus of hrGDF15 in mice with and without pre-treatment with Fc_GDF15. N=17 (12 male, 5 female) in Control and 19 in FC_GDF15 group (13 male, 6 female). Data were analysed using mixed-effect analysis, post-hoc testing comparing before and after acute GDF-15 treatment was undertaken with the Sidak test to correct for multiple testing. *P<0.05, ***P<0.001, ****P<0.0001, ns = non-significant, P>0.05.