Definition
Successful antibody for Western blot	A successful primary antibody immunodetects the target protein, and the signal observed in the WT lysate is lost in the KO lysates (Supplemental Figure 1A). The antibody does not recognize any other protein under the conditions tested.
Specific, non-selective antibody for Western blot	The primary antibody specifically recognize the target protein, as well as unrelated protein(s) (Supplemental Figure 1A).
Successful antibody for immunoprecipitation	Under the conditions used, a successful primary antibody immunocaptures the target protein to at least 10% of the starting material (Supplemental Figure 1B), which leads to an observable depletion of the target protein from the starting material.
Successful antibody for immunofluorescence	A successful primary antibody immunolocalizes the target protein by generating a fluorescence signal in WT cells that is at least 1.5-fold higher than the signal in KO cells (Supplemental Figure 1C). Signal provided by such antibody staining can be easily distinguished from unspecific background and noise.