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[Preprint]. 2023 Jun 13:2023.06.13.544803. [Version 1] doi: 10.1101/2023.06.13.544803

Figure 4. Depletion of PPKL does not affect the replication of centrosomes.

Figure 4.

Centrosome duplication of PPKLAID grown with and without auxin was monitored by IFA using antibodies against centrin1. A. IFA of parasites grown with/without IAA for 18 hours and stained with anti-IMC3, anti-Centrin1 antibodies, and DapI. B. IFA of PPKLAID parasites grown with/without IAA for 6 hours. C. quantification of dividing PPKLAID parasites treated with/without IAA for 6 hours with one or two daughters. Three biological replicates were performed, with 50 parasites counted for each replicate. The error bars represent standard deviations. D. U-ExM of one daughter containing PPKLAID parasite treated with IAA for 6 hours and stained with anti-acetylated tubulin, anti-Centrin1, NHS-ester, and Draq5. Scale bar: 5 μm.