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[Preprint]. 2023 Jun 15:2023.06.15.545170. [Version 1] doi: 10.1101/2023.06.15.545170

Figure 1: Schematic representation of the experimental workflow.

Figure 1:

Islets obtained from human cadaveric donors (n=10 total; 5M/5F) were treated with IL-1β+IFN-γ for 24 hrs. (A) Total RNA was isolated from islets and islet-derived EVs and subjected to small RNA sequencing. (B) Differentially expressed miRNAs were further validated using separate sets of islets and islet-derived EVs. (C) A panel of miRNAs selected from the top five differentially expressed EV miRNAs were validated using human circulating plasma-derived EVs from healthy controls, individuals that are AAb+, and individuals with recent onset T1D. (D) miR-155 expression patterns were analyzed in pancreatic samples from human organ donors with AAb+ and T1D and non-diabetic controls.