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[Preprint]. 2023 Jun 15:2023.06.15.545170. [Version 1] doi: 10.1101/2023.06.15.545170

Figure 3. Identification and validation of differentially expressed islet-derived miRNAs.

Figure 3.

Human islets were treated with or without 50 U/mL IL-1β and 1000 U/mL IFN-γ for 24 hours. (A) Volcano plot showing upregulated and downregulated miRNAs from human islets treated with or without cytokines. (B) Heatmap illustrates the expression of the 20 differentially expressed miRNAs identified from small RNA sequencing analysis of control and cytokine-treated human islets (n=10; 5M/5F). (C) qRT-PCR validation of upregulated and downregulated miRNAs identified from sequencing analysis of human islets. Grey filled bars represent control and blue filled bars represent cytokine treatment. n=12; 7M/5F composition for C; *p <0.05, **p<0.01, ***p<0.001.