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[Preprint]. 2023 Jun 15:2023.06.15.545170. [Version 1] doi: 10.1101/2023.06.15.545170

Figure 4. Identification and validation of differentially expressed islet-derived EV miRNAs.

Figure 4.

Human islets were treated with or without 50 U/mL IL-1β and 1000 U/mL IFN-γ for 24 hours and EVs were isolated from the culture supernatant. (A) Volcano plot showing upregulated and downregulated EV miRNAs isolated from human islets treated with or without cytokines. (B) Heatmap illustrates the expression pattern of 14 differentially expressed EV-miRNAs identified from small RNA sequencing analysis of isolated EVs from control and cytokine-treated islet culture supernatants. (N=14; 7M/7F). (C) qRT-PCR validation of upregulated and downregulated EV-miRNAs identified from small RNA sequencing analysis. Grey filled bars represent control and blue filled bars represent cytokine treatment EV data. n=8; 4M/4F for C; *p <0.05, **p<0.01, ***p<0.001.