Figure 6.

Confirmation of RNA-Seq results by qRT-PCR analysis. A. mRNA expression of six target genes, identified as significantly up- or down regulated by NO by RNA-seq, was validated using Taqman reverse transcription -quantitative PCR. A) RNA seq results, expressed as fragments per kilobase of transcript per million mapped reads [(FPKM); p< 0.05 for all genes, paired t-test]. B) Taqman RT-qPCR results, expressed as the gene of interest, normalized to GAPDH (ΔΔC(t)). *p < 0.05; Wilcoxan matched-pairs signed rank test, **p < 0.01; Wilcoxan matched-pairs signed rank test, ^##p < 0.01; Student’s t-test for matched pairs.