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[Preprint]. 2023 Jun 2:rs.3.rs-2923510. [Version 1] doi: 10.21203/rs.3.rs-2923510/v1

Figure 3. RalA deficiency in WAT increases energy expenditure and mitochondrial oxidative phosphorylation.

Figure 3

a, Regression plot of energy expenditure (EE) measured in HFD-fed Ralaf/f and RalaAKO mice (n = 5–8) during dark phase. ANCOVA test was performed using body weight (BW) as a covariate, group effect p = 0.0391. b, c, Immunoblot (b) and quantification (c) of OXPHOS proteins in iWAT of HFD mice (n = 10–13). d, e, Plasma non-esterified fatty acid (NEFA, d) and TG (e) levels in HFD-fed Ralaf/f and RalaAKO mice (n = 10–13). f, Basal oxygen consumption rate (OCR) in mitochondria measured by Seahorse. Mitochondrial fractions were isolated from primary mature adipocytes in iWAT or eWAT of HFD-fed Ralaf/f and RalaAKO mice (n = 4–5). The data (c-f) are shown as the mean ± SEM, *p < 0.05, **p < 0.01,***p < 0.001 by unpaired t-test (c-f).