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[Preprint]. 2023 Jun 2:rs.3.rs-2923510. [Version 1] doi: 10.21203/rs.3.rs-2923510/v1

Figure 5. Inhibition of RalA increases Drp1 S637 phosphorylation in white adipocytes.

Figure 5

a, Quantification of phospho-Drp1 (S637), total Drp1 and β-Tubulin immunoblotting in iWAT of HFD-fed mice (n = 10–13). b, c, Immunoblotting (b) and quantification (c) of phospho-Drp1 (S637), total Drp1, RalA, and (B-Actin in immortalized adipocytes (n = 4). Adipocytes were treated with 1 μM CL 316,243 (CL) for indicated time, d, e, Immunoblotting (d) and quantification (e) of phospho-Drp1 (S637), total Drp1 and β-Actin in human primary adipocytes (SGBS) (n = 4). Cells were pretreated with 50 μM RBC8 or DMSO for 30 min prior to treatment with 20 μM forskolin (Fsk) for indicated time, f, g, DNM1L mRNA expression is correlated with BMI (f) and HOMA (g) in human abdominal subcutaneous adipose tissue samples (n = 56). Significance in correlation was assessed by Spearman’s correlation test, h, Box-and-whisker plot of DNM1L mRNA expression in abdominal subcutaneous adipose tissues from non-obese and obese human subjects (n = 56). The data (a, c, e) are shown as the mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired t-test (c,e).