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[Preprint]. 2023 Jun 16:rs.3.rs-3026818. [Version 1] doi: 10.21203/rs.3.rs-3026818/v1

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Extended Data Fig. 3. — . (A) Huh-7 hepatocellular carcinoma cells carrying the indicated mutations in VPS35L (EV, empty vector). Immunoprecipitation of VPS35L followed by western blot for the indicated proteins is shown. (B) Immunoprecipitation of VPS35L followed by competitive elution of native complexes using HA peptide, and separation of the complexes in blue native gels. After transfer, the complexes were immunoblotted with the indicated antibodies. (C) Heatmap representation of protein-protein interaction results using proteomics. VPS35L was immunoprecipitated from the indicated Huh-7 stable cell lines (in triplicate samples) and the results are expressed as fold compared to Huh-7 control cells (darker blue depicts greater fold difference). Statistical significance is indicated in color scale (yellow indicating p<0.05, and grey indicating p>0.05). (D) Immunoprecipitation of VPS35L carrying indicated point mutations expressed in HEK293T cells and immunoblotting for the indicated proteins.