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. 2023 Jun 30;21(6):e3002133. doi: 10.1371/journal.pbio.3002133

Fig 1. Cell type profiling and major approaches.

Fig 1

A variety of multimodal techniques are used to profile cell types of the brain. A common coordinate framework (CCF) is used to map spatial distribution of types and their connectivity. Top to bottom: (A) Transcriptomic techniques, single-cell and single-nucleus (sc/sn-RNA-seq), and epigenomic (ATAC-seq), single-nucleus methylation (snmC-seq), (B) epi-retro-seq, (C) single-cell full morphology and connectivity (fMOST, BAR-seq), (D) spatial transcriptomics (MERFISH), (E) antero- and retro-grade tracing methods for morphological reconstruction. (F) Multimodal technique combining transcriptome, electrophysiology, and morphology (Patch-seq). (G) Cell type classifications are represented as taxonomies reflecting hierarchical relationships, multimodal correspondence, and cell distribution (S1 Table). (H) Transgenic mouse lines are used in selecting expressing cell types.