Fig 3. FBS activates specifically Dim and BmaDAF-12.

NIH3T3 cells were co-transfected with Gal4-DAF-12_LBD from Dim, Bma, Hco or Cel and luciferase gene reporter construct and then (A) incubated with regular or charcoal-stripped FBS for 24 hours. (B) Effects of increasing amounts of FBS on activation of Dim and Bma DAF-12 activity. The values were normalized to the empty vector-transfected cells and expressed as relative light units (RLU). Data represent the average of normalized luciferase activity and the error bars correspond to the standard deviation of three independent experiments. The significance of the effects was analyzed by unpaired Student’s t-tests using Prism 6.0 (Graph Pad Software, Inc.). **p < 0.01; ***p < 0.001.