FIGURE 2.

D + Q treatment transiently decreases infiltration of flu-specific CD8 T-Cells following primary infection. 18–20 months male mice were treated with D + Q or vehicle and infected as in Figure 1. Lungs were assayed for infiltrating flu NP-specific CD8 T-cells via flow cytometry (A). Frequency of NP-specific CD8 T-cells was measured at 10 days post infection (B). Numbers of NP-specific CD8 T-cells were also quantified (C). Expression of intracellular Granzyme B was assessed via flow cytometry (D). Data are presented as mean ± standard error of the mean (SEM) and each symbol represents a single animal. Student’s t-test was utilized for all experiments, with results being considered significant at p < 0.05. Comparisons are nonsignificant unless otherwise indicated. N = 5 per group.