| Gene (Mus musculus) |
Rps27 (eS27) |
Ensembl version 109 |
ENSMUSG00000090733 |
|
| Gene (M. musculus) |
Rps27l (eS27L) |
Ensembl version 109 |
ENSMUSG00000036781 |
|
| Gene (Homo sapiens) |
RPS27 (eS27) |
Ensembl version 109 |
ENSG00000177954 |
|
| Gene (H. sapiens) |
RPS27L (eS27L) |
Ensembl version 109 |
ENSG00000185088 |
|
| Gene (multiple species) |
Rps27 and Rps27l orthologs |
Multiple |
|
See Figure 1, Supplementary file 1 for full details |
| Strain, strain background (M. musculus) |
Rps27exon2del, C57BL/6J |
This paper |
|
See ‘Generation of genetically edited mouse lines,’ Figure 4, Supplementary file 7
|
| Strain, strain background (M. musculus) |
Rps27lexon2del, C57BL/6J |
This paper |
|
See ‘Generation of genetically edited mouse lines,’ Figure 4, Supplementary file 7
|
| Strain, strain background (M. musculus) |
Rps27Rps27l, C57BL/6J |
This paper |
|
See ‘Generation of genetically edited mouse lines,’ Figure 4, Supplementary file 7
|
| Strain, strain background (M. musculus) |
Rps27lRps27, C57BL/6J |
This paper |
|
See ‘Generation of genetically edited mouse lines,’ Figure 4, Supplementary file 7
|
| Strain, strain background (M. musculus) |
Trp53null, C57BL/6J |
Jackson Laboratories https://doi.org/10.1016/s0960-9822(00)00002-6
|
Strain #002101; RRID:IMSR_JAX:002101
|
|
| Cell line (M. musculus) |
Rps27-FLAG mESCs |
This paper |
|
See ‘Generation of CRISPR-edited mESCs,’ Figure 3, Supplementary file 7
|
| Cell line (M. musculus) |
Rps27l-FLAG mESCs |
This paper |
|
See ‘Generation of CRISPR-edited mESCs,’ Figure 3, Supplementary file 7
|
| Antibody |
Rps27 (goat polyclonal) |
Thermo Fisher |
PA5-18092; RRID:AB_10980328
|
1:10,000 |
| Antibody |
Rps27l (rabbit polyclonal) |
ProteinTech |
15871-1-AP; RRID:AB_2253903
|
1:1000 |
| Antibody |
Actb (mouse monoclonal) |
Cell Signaling |
3700S; RRID:AB_2242334
|
1:1000 |
| Antibody |
Rps3 (mouse monoclonal) |
Abcam |
ab77330; RRID:AB_1566697
|
1:1000 |
| Antibody |
FLAG (mouse monoclonal) |
MilliporeSigma |
F3165; RRID:AB_259529
|
1:1000 |
| Antibody |
Gapdh (mouse monoclonal) |
Thermo Fisher |
AM4300; RRID:AB_2536381
|
1:5000 |
| Antibody |
Trp53 (rabbit polyclonal) |
Leica Biosystems |
CM5; RRID:AB_563933
|
1:1000 |
| Sequence-based reagent |
qPCR primers |
This paper |
|
See Supplementary file 7
|
| Sequence-based reagent |
Genotyping primers |
This paper |
|
See Supplementary file 7
|
| Sequence-based reagent |
Ribosome profiling primers |
https://doi.org/10.1016/j.ymeth.2017.05.028
|
|
See also Supplementary file 7
|
| Commercial assay or kit |
NucleoSpin RNA |
Macherey-Nagel |
740955.50 |
|
| Commercial assay or kit |
PureLink RNA Mini |
Thermo Fisher |
12183018A |
|
| Commercial assay or kit |
ProteoExtract protein precipitation |
Calbiochem |
539180 |
|
| Commercial assay or kit |
Direct-Zol Microprep Kit |
Zymo |
R2060 |
|
| Commercial assay or kit |
Zymo Oligo Clean & Concentrator |
Zymo |
D4060 |
|
| Commercial assay or kit |
RiboZero Gold |
Illumina |
20020598 |
|
| Commercial assay or kit |
Zymo DNA Clean & Concentrator |
Zymo |
D4003 |
|
| Commercial assay or kit |
EdU labelling kit |
Click Chemistry Tools |
1381 |
|
| Software, algorithm |
MEGA11 |
https://doi.org/10.1093/molbev/msab120
|
|
|
| Software, algorithm |
RAxML-ng |
https://doi.org/10.1093/bioinformatics/btz305
|
|
|
| Software, algorithm |
Cutadapt version 2.4 |
https://doi.org/10.14806/ej.17.1.200
|
|
|
| Software, algorithm |
fastx_barcode_splitter.pl |
http://hannonlab.cshl.edu/fastx_toolkit/
|
|
|
| Software, algorithm |
umi_tools version 1.0.1 |
https://doi.org/10.1101/gr.209601.116
|
|
|
| Software, algorithm |
fastq_quality_filter |
http://hannonlab.cshl.edu/fastx_toolkit/
|
|
|
| Software, algorithm |
bowtie2 version 2.3.4.3 |
https://doi.org/10.1038/nmeth.1923
|
|
|
| Software, algorithm |
EdgeR |
https://doi.org/10.1093/bioinformatics/btp616
|
|
|
| Software, algorithm |
Voom |
https://doi.org/10.1186/gb-2014-15-2-r29
|
|
|
