Figure 2. Dpf2 loss results in hyperproliferation and infiltration of macrophages.

(A) Frequency of myeloid cell populations (gated on CD11b⁺). (B) Representative FACS plot of BM myeloid cell populations. (C) Percentage of EdU⁺ splenic macrophages (F4/80⁺CD11b⁺) in end-stage Dpf2^Δ/Δ and age-matched Dpf2^fl/fl mice. (D) Ki67 IHC staining of spleen and liver sections. Original magnification, ×2 and ×10. (E) Overlap between genes upregulated after Dpf2 loss in M0, M1, and M2 BMDMs (q < 0.05, fold change >1.5). Highlighted are a few of the 289 genes that were upregulated in all conditions. (F) Same as in E, but for genes that were downregulated. (G) ChEA of genes downregulated in Dpf2^Δ/Δ compared with Dpf2^fl/fl BMDMs. (H) Hallmark GSEA of gene expression programs enriched in Dpf2^Δ/Δ BMDMs. NES, normalized enrichment score; DN, downregulated; UP, upregulated. Data represent the mean ± SD. P values were calculated using a 2-tailed, unpaired Student’s t test.