Figure 8. Pharmacological stimulation of NRF2 can restore gene expression and prolong survival of the Dpf2^Δ/Δ mice.

(A) Kaplan-Meier survival curves of mice treated with vehicle or CDDO-Im. Treatment was interrupted after 85 days (dashed line). A log-rank (Mantel-Cox) text was performed to determine significant differences in the survival of Dpf2^Δ/Δ mice treated with vehicle or CDDO-Im. (B and C) Expression of Dpf2 and Nrf2 (B), and NRF2 target genes (C) in LK cells from Dpf2^fl/fl and Dpf2^Δ/Δ mice treated with vehicle or CDDO-Im. P value for vehicle-treated versus CDDO-Im–treated Dpf2^Δ/Δ mouse data are indicated below each plot. (D) sCD25 in plasma from the indicated groups of mice. (E) PB counts in mice treated for 2 weeks with vehicle or CDDO-Im. (F) Flow cytometric analyses of splenic cell populations. Data represent the mean ± SEM. P values were calculated using 2-way ANOVA.