Fig. 3.

Immunohistochemical detection of PHT2/SLC15A3 and PHT1/SLC15A4 transporters in serial cross sections of the longissimus dorsi (LD) and semitendinosus (ST) muscles. Serial sections were stained with anti-SLC15A3 and anti-SLC15A4 antibodies (left panels, green staining) or with a histochemical reaction based on the NADH-tetrazolium reductase activity (right panels) to identify white glycolytic (W), intermediate (I) and red oxidative (R) muscle fibres. SLC15A3 and SLC15A4 signals are found at the periphery of muscle fibres, with a much stronger signal intensity present in red oxidative fibres for both muscles. A diffuse cytosolic staining is also present in all fibre types of the LD and RH muscles, with a stronger intensity found in red oxidative fibres. Scale bar = 200 µm