Table 1.
List of primary antibodies used in this work
| Name | Antigen | Type | Dilution | Origin |
|---|---|---|---|---|
| Antibodies used for immunofluorescence microscopy: | ||||
| RR90 | filamin-A/C | mouse mAb IgA | 1:30—1:50 | van der Ven et al. (2000) |
| FILIP1 | FILIP1 | rabbit pAb | 1:50—1:150 | Atlas antibodies |
| EB3 (7) | EB3 | mouse mAb IgG1 | 1:20 | Santa Cruz Biotechnology |
| Acetylated tubulin (Lys40) | acetylated alpha-tubulin | mouse mAb IgG1 | 1:1,000 | Proteintech |
| YL1/2 | tyrosylated alpha-tubulin | rat mAb IgG2a | 1:50 | Wehland et al. (1983) |
| E7 | beta-tubulin | mouse mAb IgG1 | 1:20 | DSHB |
| T12 | titin (Z-disc) | mouse mAb IgG1 | 1:20 | Fürst et al. (1988) |
| Antibodies used for western blot analysis: | ||||
| FLNc d16-20 | filamin-C | rabbit pAb | 1:10,000 | Chevessier et al. (2015) |
| FILIP1 | FILIP1 | rabbit pAb | 1:250 | Reimann et al. (2020) |
| TUBA1B (clone 6-11B-1) | acetylated tubulin alpha 1b | mouse mAb IgG2b | 1:2,000 | BioLegend |
| YL1/2 | tyrosylated alpha-tubulin | rat mAb IgG2a | 1:500 | Invitrogen |
| EB3 | EB3 | rabbit pAb | 1:2,000 | Proteintech |
| GAPDH (clone 5C6) | GAPDH | mouse mAb IgG1 | 1:1,000 | Calbiochem |