Table 2.
List of secondary antibodies used in this work
| Name | Donor | Directed against | Dilution | Supplier |
|---|---|---|---|---|
| Antibodies used for immunofluorescence microscopy: | ||||
| GAMouse IgA Alexa Fluor 488 | goat | mouse IgA | 1:30 | Southern Biotechnology Associates |
| GARabbit Alexa Fluor 594plus | goat | rabbit Ig | 1:300 | ThermoFisher |
| GAMouse IgG1 Cy5 | goat | mouse IgG1 | 1:100 | Jackson ImmunoResearch |
| GARat Alexa Fluor 405plus | goat | rat Ig | 1:150 | ThermoFisher |
| GARabbit Cy3 | goat | rabbit Ig | 1:300 | Jackson ImmunoResearch |
| Antibodies used for Western blot analysis: | ||||
| GAMouse-HRP | goat | mouse Ig | 1:10,000 | Jackson ImmunoResearch |
| GARabbit-HRP | goat | rabbit Ig | 1:30,000 | Jackson ImmunoResearch |
| GARat-HRP | goat | rat Ig | 1:10,000 | Jackson ImmunoResearch |
| GARabbit IRDye800 | goat | rabbit Ig | 1:10,000 | LI-COR |
| GAMouse IRDye680 | goat | mouse Ig | 1:5,000 | LI-COR |
HRP horse radish peroxidase, IRDye infrared dye