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. 2023 Jun 30;80(7):193. doi: 10.1007/s00018-023-04841-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Characterization of mouse brain-derived EVs. α. Negatively stained TEM of EVs from fraction d. Scale bar 200 nm (left panel) and 100 nm (right panel). b. Western blotting of brain-derived EVs isolated from fraction d (lane 1), EVs derived from primary neuronal culture (lane 2), and brain tissue lysate (lane 3) (upper panel). 15 μg total protein loaded. Western blotting of different fractions (b, c, d) against TSG101, VDAC, and GAPDH. Cell lysate from HEK-293 cells was used as a positive control (LE: low exposure; HE: high exposure). 30 μg total protein loaded. f. Representative NTA distribution profile of EVs (fraction c and d, as well as DiI-stained fraction d), including size distribution plots and mean size of each peak