Table 3.

Haematological findings in male Swiss Albino mice treated with ruthenium biochanin-A complex for 28 days repeated-dose oral sub-acute toxicity study

Parameters	Control	ruthenium biochanin-A (50 mg/kg)	Ruthenium biochanin-A (100 mg/kg)	Ruthenium biochanin-A (200 mg/kg)	Ruthenium biochanin-A (400 mg/kg)
Haemoglobin (Gm%)	12.4 ± 0.008	12.40 ± 0.008	12.38 ± 0.009	12.36 ± 0.005*#	12.41 ± 0.006α
Total RBC count (×106/µL)	4.31 ± 0.001	4.31 ± 0.001	4.32 ± 0.002	4.31 ± 0.003	4.30 ± 0.001
WBC count (×103/µL)	5.43 ± 0.001	5.49 ± 0.004*	5.52 ± 0.002*	5.76 ± 0.003*#$	15.12 ± 0.001*#$α
Platelet count (×105/µL)	6.92 ± 0.005	6.91 ± 0.002	7.07 ± 0.003*#	7.11 ± 0.002*#$	6.87 ± 0.003*$α
MCV (fL)	90.86 ± 0.004	88.92 ± 0.008*#	91.88 ± 0.005*#	90.88 ± 0.006#$	90.95 ± 0.009*#$α
MCH (pg)	28.87 ± 0.003	28.75 ± 0.005*#	27.85 ± 0.005*	28.77 ± 0.006*$	28.88 ± 0.004#$α
MCHC (%)	2.13 ± 0.004	31.12 ± 0.005*	32.13 ± 0.006*#	32.13 ± 0.006*#	33.14 ± 0.004*#$α
Neutrophil (%)	55.03 ± 0.006	45.34 ± 0.006*	46.73 ± 0.006*#	55.07 ± 0.008*#$	48.14 ± 0.007*#$α
Eosinophil (%)	2.45 ± 0.01	2.55 ± 0.005*	3.02 ± 0.005*#	3.46 ± 0.006*#$	2.58 ± 0.006*#$α
Basophil (%)	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
Lymphocyte (%)	45.05 ± 0.12	37.05 ± 0.01*	20.64 ± 0.07*#	40.17 ± 0.007*#$	42.76 ± 0.009*#$α
Monocyte (%)	3.18 ± 0.003	3.18 ± 0.005	4.03 ± 0.013*#	3.88 ± 0.006*#$	3.56 ± 0.006*#$α

The values were depicted as mean ± SEM (n = 6). The values were analyzed through one-way ANOVA associated with Tukey’s multiple comparison test

MCV mean corpuscular volume, MCH mean corpuscular haemoglobin, MCHC mean corpuscular haemoglobin concentration, RBC red blood cell, WBC white blood cell

*Significant difference as compared to the control group (p < 0.05)

^#Significant difference as compared to the 50 mg/kg complex treated group (p < 0.05)

^$Significant difference as compared to the 100 mg/kg complex treated group (p < 0.05)

^αSignificant difference as compared to the 200 mg/kg complex treated group (p < 0.05)