Table 5.
Serum biochemistry findings in male Swiss Albino mice treated with ruthenium biochanin-A complex for 28 days repeated-dose oral sub-acute toxicity study
| Parameters | Control | Ruthenium biochanin-A (50 mg/kg) | Ruthenium biochanin-A (100 mg/kg) | Ruthenium biochanin-A (200 mg/kg) | Ruthenium biochanin-A (400 mg/kg) |
|---|---|---|---|---|---|
| Aspartate amino transferase (AST) | 50.84 ± 0.008 | 52.66 ± 0.012* | 52.66 ± 0.012# | 56.35 ± 0.012*#$ | 111.80 ± 0.010*#$α |
| Alanine amino transferase (ALT) | 39.36 ± 0.007 | 38.38 ± 0.009* | 38.95 ± 0.011*# | 40.05 ± 0.014*#$ | 100.10 ± 0.010*#$α |
| Alkaline phosphatase (ALP) | 77.34 ± 0.013 | 65.75 ± 15.90* | 79.05 ± 0.012*# | 60.75 ± 0.012*#$ | 102.10 ± 0.014*#$α |
| Total protein (g/dL) | 5.74 ± 0.011 | 6.35 ± 0.014* | 6.35 ± 0.008* | 5.94 ± 0.010*#$ | 2.45 ± 0.012#$α |
| Albumin (g/dL) | 3.95 ± 0.006 | 3.86 ± 0.013* | 3.74 ± 0.009*# | 3.56 ± 0.009*#$ | 5.85 ± 0.012*#$α |
| Globulin (g/dL) | 3.94 ± 0.009 | 3.85 ± 0.011* | 3.56 ± 0.011*# | 3.65 ± 0.011*#$ | 3.95 ± 0.007#$α |
| Total bilirubin (mg/dL) | 0.43 ± 0.007 | 0.44 ± 0.015 | 0.66 ± 0.011*# | 0.53 ± 0.009*#$ | 1.95 ± 0.007*#$α |
| Blood urea nitrogen (mg/dL) | 16.17 ± 0.009 | 17.86 ± 0.009* | 16.37 ± 0.007*# | 16.15 ± 0.012#$ | 29.34 ± 0.009*#$α |
| Creatinine (mg/dL) | 0.65 ± 0.011 | 0.65 ± 0.013 | 0.66 ± 0.007 | 0.67 ± 0.008 | 1.85 ± 0.013*#$α |
The values were depicted as mean ± SEM (n = 6). The values were analyzed through one-way ANOVA associated with Tukey’s multiple comparison test
*Significant difference as compared to the control group (p < 0.05)
#Significant difference as compared to the 50 mg/kg complex treated group (p < 0.05)
$Significant difference as compared to the 100 mg/kg complex treated group (p < 0.05)
αSignificant difference as compared to the 200 mg/kg complex treated group (p < 0.05)