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Summary of the radiolabeling conditions
| Condition 1 | Condition 2 | Condition 3 | |
|---|---|---|---|
| MilliQ water | 10 μL | 10 μL | |
| MilliQ water containing Kolliphor® HS 15 (2.0 mg/mL) | 90.5 μL | ||
| TRIS buffer containing the peptide (0.1 M, pH 9) | 75 μL | 75 μL | |
| Ascorbate buffer (1.0 M, pH 5.8) | 50 μL | 50 μL | |
| l-melatonin (0.5 M) | 10 μL | ||
| Gentisic/ascorbic acids (50 mM) | 10 μL | ||
| Sodium acetate (2.5 M, pH 8) | 1 μL | ||
| Ethanol | 10 μL | ||
| Molar activity | 50 kBq/nmol | 100 kBq/nmol | 100 kBq/nmol |
| DTPA (4 mM) | 5 μL | 5 μL | 5 μL |
