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. 2023 Jun 30;14:3867. doi: 10.1038/s41467-023-39563-6

Fig. 3. The effect of actomyosin contractility, actin cytoskeleton integrity and LINC complexes on nuclear force transduction.

Fig. 3

a sFRET efficiency images of Lamin-SS and Lamin-TM after cell contractility inhibition (Y-27632, 50 µM, 1 h). Scale bars, 20 µm. b Quantified sFRET (mean ± SEM) of Lamin-SS and Lamin-TM sensors after ROCK-inhibition (n = 5 fields, 3 biological replicates). Ordinary one-way ANOVA Tukey’s multiple comparisons (for Lamin-SS (**) p = 0.005 and Lamin-TM (ns) p = 0.4132). c Lamin-SS riFRET imaging during ROCK-inhibition (Y-27632, 50 µM, added at time point 0 min). Scale bar 20 µm. d Quantified relative change (mean ± SEM) in Lamin-SS riFRET ratio during ROCK-inhibition (n = 152 cells, two biological replicates, black and gray). e Disruption of LINC complexes by dominant-negative KASH (DN-KASH) expression (induction for 24 h). sFRET efficiency images of Lamin-SS and Lamin-TM after LINC disruption. Scale bars, 20 µm. f Quantified sFRET (mean ± SEM) of Lamin-SS and Lamin-TM after LINC complex disruption (n = 10 fields, two biological replicates). Ordinary one-way ANOVA Tukey’s multiple comparisons (for Lamin-SS (**) p = 0.004 and Lamin-TM (ns) p = 0.1722).