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. 2023 Jun 30;13:10643. doi: 10.1038/s41598-023-37347-y

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Optimization of the blocking buffer and conditions for an enhanced strip based upon the blocking buffer and the immersion time for the same test sample, (A) 50 mM boric acid and 0.3% skim milk and 15 min. immersion time, (B) 50 mM boric acid and 0.3% skim milk and 20 min immersion time), (C) 50 mM boric acid and 0.3% skim milk and 10 min immersion time. (A) Showed the most identified control and test line (strongest red color).