Figure 2.

CAR-T_RM cells possess a core resident memory transcriptomic signature

Bulk RNA-seq experiments were conducted using CD8⁺ CAR-T_CONV and CAR-T_RM cells.

(A) Principal-component analysis was carried out with RNA expression data.

(B) Gene set enrichment analysis (GSEA) using gene sets associated with resident memory T cells.

(C) Heatmap showing differentially expressed genes from CD69⁺CD103⁺ and CD69⁻CD103⁻CD8⁺ T cell signatures.

(D) Heatmap displaying expression of transcription factor genes known to promote and suppress resident memory T cell differentiation.

(E) GSEA (hypoxia pathways: MSigDB C2).

(F) Pathway analysis incorporating genes upregulated in CAR-T_RM-cells using Enrichr.

(G) GSEA analysis (migration pathways: MSigDB C2). GSEA with gene signatures of tumor-infiltrating T cells associated with response to ICB. Bulk RNA-seq experiments were conducted using CAR T cells derived from n = 3 distinct healthy donors, serving as biological replicates.