Figure 3.

TGF-β-generated CAR-T_RM cells transcriptionally resemble stem-like T_RM cells

(A) UMAP imbedding of CD8⁺CAR T cell (top, CD8 subclusters; middle, CAR T cell samples; bottom, cell cycle). Frequencies of three CD8⁺ clusters in CAR-T_CONV and CAR-T_RM cells are shown.

(B) Bubble plot displaying expression levels of marker genes in each CD8⁺ subcluster.

(C) Violin plots showing expression of KLF2 and ITGAE.

(D) CD69⁻CD103⁻ and CD69⁺CD103⁺CD8⁺ T cell signature scores calculated for CAR-T_CONV and CAR-T_RM cells.

(E) A UMAP plot showing CAR T cell sample origin in TCF7⁺CD8⁺T cells.

(F) Expression of resident memory (ITGAE, BHLHE40, CCL5) and early memory (IL7R) markers in TCF7⁺CD8⁺ T cells.

(G) Module score comparison for CD69⁻CD103⁻ and CD69⁺CD103⁺ CD8⁺ T cell signatures between CAR-T_CONV and CAR-T_RMTCF7⁺CD8⁺ T cells.