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. 2023 Jun 16;10:1112573. doi: 10.3389/fmed.2023.1112573

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Copyright © 2023 Hakuno, Janson, Trietsch, de Graaf, de Jonge-Muller, Crobach, Harryvan, Boonstra, Dinjens, Slingerland and Hawinkels.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of endoglin by SCC cell lines. Expression by 10 ESCC cell lines, where endoglin expression by TE01 ( p < 0.0001) and TE15 (p < 0.003) significantly differ from all other cell lines (A), OSC-19 and FaDu show significantly different endoglin expression (p = 0.0002) (B), as is also detected in the three VSCC cell lines (*p = 0.0123, **p = 0.0025, ***p = 0.0001) with different morphologies (conventional—VC415-C and VC704; spindle—VC415-S) (C). Endoglin protein levels were determined via western blot (D) and ELISA. Endoglin protein expression by TE01 significantly differs from all other cell lines—(p ≤ 0.0018) (E). Image for western blot analysis is a representative image of n = 2–3 independent experiments.