Table 1.

Six marker FFPE panel for imaging mass spectrometry (Hyperion).

Target	Clone	Metal	Time	Temperature	Dilution
Endoglin + 2nd AB (biotin)	BAF1097	147 Sm	indirect O/N + 1 h	4° C + rT	1:50 + 1:100
Pan-cytokeratin	AE1/AE3	148 Nd	5 h	rT	1:100
p53	7F5	163 Dy	O/N	4° C	1:100
CD45	D9M8I	145 Nd	O/N	4° C	1:50
CD68	D4B9C	157 Gd	O/N	4° C	1:100
Vimentin	D21H3	143 Nd	5 h	rT	1:200

AB, antibody; O/N, overnight; and rT, room temperature.