Figure 4.
Characterization of macrophage populations in LUADs
(A) Scatterplots of mean expression of M1 and M2 marker genes in four types of macrophages from tNS (up) and tS (bottom).
(B–E) Violin plots with included boxplot of the M1/M2 scores in MDM (B and D) and TRM (C and E). Two-sided Wilcoxon signed-rank test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Asterisks represent the significance of differences in the following comparisons: all tumor tissues vs. all normal lung tissues (brown), nS vs. nNS (black), tS vs. tNS (red), and tNS vs. nNS and tS vs. nS (green).
(F and H) Dot plot of mean expression of M2 signature (F) and anti-inflammatory signature (H) in TRM.
(G, I, and J) Violin plots with included boxplot of anti-inflammatory scores in TRM (G) and AP scores in TRM (I) and MDM (J). Two-sided Wilcoxon signed-rank test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Asterisks represent the significance of differences in the following comparisons: all tumor tissues vs. all normal lung tissues (brown), nS vs. nNS (black), tS vs. tNS (red), and tNS vs. nNS and tS vs. nS (green).
(K) Violin plots with included boxplot of M1/M2 scores, anti-inflammatory scores, and AP scores in SPP1hi MDM from tNS and tS. Two-sided Wilcoxon signed-rank test, ∗∗∗p < 0.001
(L) Violin plots with included boxplot of PD-L1 expression in SPP1hi MDM from tNS and tS. Two-sided Wilcoxon signed-rank test, ∗∗∗p < 0.001