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. 2023 Jun 9;4(6):101072. doi: 10.1016/j.xcrm.2023.101072

Figure 2.

Figure 2

FAM3D deficiency ameliorates AngII-induced hypertension and related vascular pathologies

(A) SBP of WT and FAM3D−/− mice was measured by the tail-cuff method during AngII infusion for 14 days. n = 6–8. Data are represented as mean ± SEM. Repeated-measures analysis using a mixed-effects model, ∗p < 0.05.

(B–D) The SBP (B), DBP (C), and MAP (D) of WT and FAM3D−/− mice were measured by radiotelemetry during AngII infusion for 14 days. n = 5–6. Data are represented as mean ± SEM. Repeated-measures analysis using a mixed-effects model, ∗p < 0.05.

(E and F) 24-h recordings of SBP (E) and DBP (F) in WT and FAM3D−/− mice was measured by radiotelemetry following 7 days of AngII infusion. n = 3–5. Data are represented as mean ± SEM. Repeated-measures analysis using a mixed-effects model, ∗p < 0.05.

(G) Pulse wave velocity (PWV) in aortas from WT and FAM3D−/− mice treated with saline or AngII for 14 days. n = 5–6. Data are represented as mean ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons test, ∗p < 0.05.

(H) Representative Masson staining and quantification of adventitial collagen deposition in thoracic aortas from WT and FAM3D−/− mice treated with saline or AngII for 14 days. Scale bar, 100 μm. n = 5–6. Data are represented as mean ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons test, ∗p < 0.05.

(I) Flow cytometric analysis of CD45+ cells in aortas from WT and FAM3D−/− mice treated with saline or AngII for 14 days. n = 4–6. Data are represented as mean ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons test, ∗p < 0.05.