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. 2023 Jun 9;4(6):101072. doi: 10.1016/j.xcrm.2023.101072

Figure 7.

Figure 7

The FAM3D-neutralizing antibodies ameliorate AngII- and DOCA-salt-induced hypertension in mice

(A) Schematic diagram showing the administration of the FAM3D-neutralizing antibodies (6D7) or mouse IgG (100 μg/30 g) to C57BL/6 mice during 14 days of AngII infusion.

(B) Representative western blot and quantification of monomer/dimer eNOS ratios in the aortas of C57BL/6 mice that were intraperitoneally injected with mouse IgG or 6D7 during AngII infusion for 14 days. n = 5. Data are represented as mean ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons test, ∗p < 0.05.

(C) Representative DAF-FM DA en face staining and quantification of NO in ECs from the thoracic aortas of C57BL/6 mice that were intraperitoneally injected with mouse IgG or 6D7 during AngII infusion for 14 days. n = 3–4. Data are represented as mean ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons test, ∗p < 0.05.

(D) SBP of C57BL/6 mice that were intraperitoneally injected with mouse IgG or 6D7 during AngII infusion for 14 days. n = 6–7. Data are represented as mean ± SEM. Repeated-measures analysis using a mixed-effects model, ∗p < 0.05.

(E) Schematic diagram showing the administration of the 6D7 or mouse IgG (100 μg/30 g) to C57BL/6 mice during 21 days of DOCA-salt treatment.

(F) Representative western blot and quantification of monomer/dimer eNOS ratios in the aortas of C57BL/6 mice that were intraperitoneally injected with mouse IgG or 6D7 during DOCA-salt treatment for 21 days. n = 5. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗p < 0.05.

(G) Representative DAF-FM DA en face staining and quantification of NO in ECs from the thoracic aortas of C57BL/6 mice that were intraperitoneally injected with mouse IgG or 6D7 during DOCA-salt treatment for 21 days. n = 4. Data are represented as mean ± SEM. Unpaired Student’s t test, ∗p < 0.05.

(H) SBP of C57BL/6 mice that were intraperitoneally injected with mouse IgG or 6D7 during DOCA-salt treatment for 21 days. n = 6. Data are represented as mean ± SEM. Repeated-measures analysis using a mixed-effects model, ∗p < 0.05.