REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat anti-FAM3D	R&D Systems	Cat# MAB2869; RRID: AB_2262535
Alexa Fluor 647 anti-mouse CD144 (VE-cadherin)	BioLegend	Cat# 138005; RRID: AB_10568319
Mouse anti-CD31	Santa Cruz Biotechnology	Cat# sc-376764; RRID: AB_2801330
Alexa Fluor 488 donkey anti-goat IgG	Life Technologies	Cat# A32814; RRID: AB_2762838
Alexa Fluor 555 goat anti-mouse IgG	Life Technologies	Cat# A21422; RRID: AB_141822
Fluorescein isothiocyanate (FITC) anti-mouse CD45	BioLegend	Cat# 103107; RRID: AB_312972
Mouse anti-eNOS	Santa Cruz	Cat# sc-376751; RRID: AB_2832203
Chemicals, peptides, and recombinant proteins
Angiotensin II	Sigma–Aldrich	A9525
Deoxycorticasterone acetate (DOCA)	Innovative Research	M-121
DAF-FM DA fluorescent dyes	Beyotime Institute of Biotechnology	S0019
DHE fluorescent dyes	Beyotime Institute of Biotechnology	S0063
DCFH-DA fluorescent dyes	Beyotime Institute of Biotechnology	S0033S
Osmotic minipumps	Alzet Corporation	Model 2001 or Model 2002
Acetylcholine	Sigma-Aldrich Chemical	60-31-1
L-NAME	Sigma-Aldrich Chemical	51298-62-5
Sodium nitroprusside	Sigma-Aldrich Chemical	13755-38-9
Phenylephrine	Sigma-Aldrich Chemical	P1240000
Apamin	MedChemexpress	HY-P0256
TRAM-34	MedChemexpress	HY-13519
Indomethacin	MedChemexpress	Hy-14397
Critical commercial assays
CircuLex Human FAM3D ELISA Kit	Marine Biological Laboratory	CY-8201
Mouse FAM3D ELISA Kit	CUSABIO	CSB-EL008229MO
Trichrome stain Kit	Solarbio Life Sciences	G1340
Nitrate/nitrite colorimetric assay kit	Beyotime Institute of Biotechnology	S0021
Experimental models: Organisms/strains
Mouse: C57BL/6 mice, FAM3D−/−	He et al., 201820	https://doi.org/10.1161/ATVBAHA.118.311289
Rat: Wistar-Kyoto rats	Vital River	21005A
Rat: spontaneously hypertensive rats	Vital River	21006A
Software and algorithms
GraphPad Prism 9.0 software	Graphpa by dotmatics	https://www.graphpad.com/
Odyssey infrared imaging system software	LI-COR Biosciences	https://www.licor.com/
QuantStudio Design & Analysis Software	Thermo Fisher Scientific	https://www.thermofisher.cn/cn/zh/home/life-science/pcr/real-time-pcr/real-time-pcr-instruments/quantstudio-3-5-real-time-pcr-system/quantstudio-3.html
LAS X software	Leica	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/
LabChart 8 Reader software	ADINSTRUMENTS	https://www.adinstruments.com/products/labchart-reader
FlowJo 7 software	FLOWJO	https://www.flowjo.com/
ImageJ software	ImageJ	https://github.com/