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. 2022 Aug 10;78(6):617–630. doi: 10.1136/thoraxjnl-2021-218194

Figure 3.

Figure 3

MSC EVs regulate pro-inflammatory cytokine secretion and pSTAT5 and SOCS1 expression in MDMs exposed to plasma samples from patients with ARDS in vitro. (A) Levels of TNF-α and IL-8 in MDMs supernatants (measured by ELISA) after stimulation with pooled healthy or hypo-inflammatory or hyper-inflammatory ARDS plasma for 24 hours (n=3–5). (B) Immunoblot of pSTAT5, STAT5, SOCS1 and β-actin protein expression in human MDM lysates after MDM exposure to healthy or ARDS plasma for 24 hours (n=4). Immunoblots were quantified by densitometry and normalised using total STAT5 expression for pSTAT5 or β-actin expression for SOCS1. Data are represented as mean±SD. Kruskal-Wallis test with post-hoc Dunn’s test (A, B). ARDS, acute respiratory distress syndrome; CM, conditioned medium; IL, interleukin; EV, extracellular vesicles; IL, interleukin; MDM, monocyte-derived macrophages; MSC, mesenchymal stromal cells; pSTAT, phosphorylated STAT; SOCS1, suppressor of cytokine signalling 1; STAT, 5, signal transducers and activators of transcription 5; TNF, tumour necrosis factor.