Skip to main content
. 2022 Aug 10;78(6):617–630. doi: 10.1136/thoraxjnl-2021-218194

Figure 7.

Figure 7

Overexpression of miR181a in MSC-EVs improves the therapeutic efficacy of MSC-EVs in the in vivo model of LPS-induced lung injury. (A) Total protein concentrations in the BALF samples 24 hours after LPS administration (n=6 mice per experimental group, n=1 sham control). (B) Total leucocyte counts (left graph, n=6 mice per experimental group, n=1 sham control) and neutrophil cell counts (n=1–3, right graph) in the BALF samples). (C) Representative images of the BALF cytospin preparations, 24 hours after LPS administration demonstrating cell recruitment to the airspaces. Images were taken using Leica Epifluorescence DM5500 microscope (objective lens original magnification ×20). (D) BALF levels of TNF-α and keratinocyte-derived chemokine (KC, murine analogue of interleukin-8) (n=6 mice per experimental group, n=1 sham control). (E) Representative confocal microscopy of alveolar macrophages, isolated from BALF 24 hours after LPS administration and stained with anti-CD68 and anti- pSTAT5 Ab. Arrows indicate co-localisation of CD68 and pSTAT5. The images were taken using Leica SP8 confocal microscope with a 100× oil-immersion objective (n=3, scale bar=50 µm). Quantitative fluorescence intensity was analysed by Image J software (MFI-pSTAT5). (F) Representative confocal microscopy of alveolar macrophages stained with anti-CD68 and anti-SOCS1 Ab. Arrows indicate co-localisation of CD68 and SOCS1. The images were taken using Leica SP8 confocal microscope with a 100× oil-immersion objective (scale bar=50 µm). Quantitative fluorescence intensity was analysed by Image J software (MFI-SOCS1). Data are represented as mean±SD. Kruskal-Wallis test with post-hoc Dunn’s test (A, B, D, E, F). Ab, antibody; BALF, bronchoalveolar lavage fluid; DAPI, 4',6-diamidino-2-phenylindol; EVs, extracellular vesicles; LPS, lipopolysaccharide; MFI, mean fluorescent intensity; MSCs, mesenchymal stromal cells; PBS, phosphate-buffered saline; pSTAT, phosphorylated STAT; SOCS1, suppressor of cytokine signalling 1; STAT, signal transducers and activators of transcription ;TNF, tumour necrosis factor.