Figure 1.

Acute pancreatitis (AP) is associated with changes of the intestinal microbiota composition. (A) Faecal samples from colon, caecum and duodenum were collected from C57Bl/6 mice with AP (n=24) and from untreated control animals Con (n=10). Isolated DNA was analysed by 16S rRNA gene sequencing. The microbiota composition and AP-associated changes of the taxonomic units with the highest abundance are illustrated by a stacked bar graph. (B) Principal coordinate analysis illustrates the changes of gut microbiome between untreated controls and AP mice. (C, D) Shannon-Diversity Index (C) and richness of observed species (D) demonstrate significant impact of AP on the duodenal microbiome composition. (E–G) The bar graph illustrates changes of the most abundant taxa in colonic (E), caecal (F) and duodenal samples (G) of AP (red) and control mice (grey). Facultative pathogenic were marked in red, beneficial commensal bacteria were marked in green. Statistical significance was determined by unpaired Student’s t-test and Kruskal-Wallis test followed by a Dunn’s multiple comparisons test to analyse differentially abundant taxa in colon, caecum and duodenum samples. Significance levels of p<0.05 are marked by an asterisk.