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. 2023 Jun 30;11(6):e006287. doi: 10.1136/jitc-2022-006287

Figure 1.

Figure 1

Expression and functionality of MCTs on CAR T cells. Non-transduced or αCD19-CAR T cells were cultured with Raji or Raji-CD19KO cells for 24 hours. Representative histogram of (A) MCT-1, (B) CD147 and (C) MCT-4 expression on CAR T cells measured by flow cytometry. Intracellular lactate relative to vehicle control on cells cultured with (D) AZD3965 or (E) AR-C155858. (F) Representative histogram and (G) relative MFI of pH Rodo Green AM in Raji cells and activated CAR T cells after 48 hours of activation. (H) Fold expansion of CAR T cells after 4 and 7 days of culture with 100 nM of MCT-1 inhibitors. (I) Expression of annexin V on CAR T cells after 48 hours of activation measured by flow cytometry. Pooled data of two or three independent experiments, n=4–7 healthy donors per group. Bars are the mean±SD. *p<0.05, **p<0.01, ns, non-significant by Friedman one-way analysis of variance. CAR, chimeric antigen receptor; MCT, monocarboxylate transporters; NT, non-transduced; MFI, mean fluorescence intensity.