Figure 3.

MCT-1 inhibition improved CAR T cells mediated cytotoxicity against B-cell lymphoma cell lines. MCT-1 inhibitors were added to non-transduced or αCD19-CAR T cells in culture with target cells for 48 hours. (A) Representative contour plot and count of Raji cells. (B) Percentage of live Raji cells cultured with CAR T cells at different effector:target ratios (E:T) ratios. (C) Representative contour plot and the total number of NALM-6 cells. (D) Percentage of live NALM-6 cells cultured with CAR T cells at different E:T ratios. Pooled data of three independent experiments, n=7–8 healthy donors per group. Bars are the mean±SEM. Expression of activation markers (E) 4-1BB, (F) OX40, (G) CD69 and (H) granzyme B on activated CAR T cells with Raji cells or Raji-CD19^KO for 24 hours. (I) IFN-γ and (G) IL-2 production was measured by ELISA after 48 hours. Pooled data of three independent experiments, n=7 healthy donors per group. Bars are the mean±SD. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns, non-significant by Friedman one-way analysis of variance. CAR, chimeric antigen receptor; IFN, interferon; IL, interleukin; MCT, monocarboxylate transporters; NT, non-transduced.