Fig. 2.

Differentiation of GCSCs. GCSCs derived from MKN-45 cell line were cultured at 5×10³ cells in a 60-mm² cell culture dish in DMEM/F12 supplemented with 10% FBS and 1 µM of dexamethasone and 10 µg/mL of insulin as adipogenesis-inducing agents and 100 nM of staurosporine as a neural-inducing agent. (A) Untreated GCSCs; (B) Oil Red O staining of GCSCs treated with dexamethasone and insulin for two weeks, and lipid vacuoles in adipose cells were detected under a microscope (AX-71, Olympus, Japan); (C) GCSCs treated with staurosporine for 24 h, and neural cells were detected under the same microscope (magnification 200×).